Composite

Part:BBa_K1300009:Design

Designed by: Ellen Jorgensen, Katty Wu   Group: iGEM14_StuyGem_NYC   (2014-06-19)


araC gene + pBAD + cI repressor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1879
    Illegal SapI site found at 961


Design Notes

We designed the construct so that araC would be constantly made. Thereby, repressing the pBAD promoter. In order for these two parts to be on the construct, we made sure that the araC gene and promoter (BBa_K808000) was on the opposite strand of DNA. This would cause the polymerase to transcribe the araC in the other direction. Since the araC gene and promoter are on the opposite strand, the translational GFP in front of the pBAD construct (BBa_K611014) would not be expressed. This renders the GFP irrelevant so that the construct focuses on the araC and pBAD.

Source

Built from existing parts in the registry though BioBrick assembly. 

araC gene [1] & pBAD + cI repressor [2]

References